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Tokiwa Inc liver cell biology
Liver Cell Biology, supplied by Tokiwa Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/liver+cell+biology/human+liver+cancer+cell+lines/pmc05196935-7-211-43
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Article Title: Isolation of Hepatic Progenitor Cells From Human Liver With Cirrhosis Secondary to Biliary Atresia Using EpCAM or Thy-1 Markers
Article Snippet: Published online 2012 Jun 15. doi: [ 10.3727/215517912X639441 ] PMCID: PMC5196935 PMID: 28058189 Taisuke Yamazaki , * Shin Enosawa , † Mureo Kasahara , ‡ Akinari Fukuda , ‡ Seisuke Sakamoto , ‡ Takanobu Shigeta , ‡ Atsuko Nakazawa , § and Takayoshi Tokiwa * Taisuke Yamazaki *Department of Liver Cell Biology, Kohno Clinical Medicine Research Institute, Shinagawa-ku, Tokyo, Japan Find articles by Taisuke Yamazaki Shin Enosawa †Division for Advanced Medical Science, National Center for Child Health and Development, Tokyo, Japan Find articles by Shin Enosawa Mureo Kasahara ‡Division of Surgery, National Center for Child Health and Development, Tokyo, Japan Find articles by Mureo Kasahara Akinari Fukuda ‡Division of Surgery, National Center for Child Health and Development, Tokyo, Japan Find articles by Akinari Fukuda Seisuke Sakamoto ‡Division of Surgery, National Center for Child Health and Development, Tokyo, Japan Find articles by Seisuke Sakamoto Takanobu Shigeta ‡Division of Surgery, National Center for Child Health and Development, Tokyo, Japan Find articles by Takanobu Shigeta Atsuko Nakazawa §Department of Clinical Pathology, National Center for Child Health and Development, Tokyo, Japan Find articles by Atsuko Nakazawa Takayoshi Tokiwa *Department of Liver Cell Biology, Kohno Clinical Medicine Research Institute, Shinagawa-ku, Tokyo, Japan Find articles by Takayoshi Tokiwa Author information Copyright and License information Disclaimer *Department of Liver Cell Biology, Kohno Clinical Medicine Research Institute, Shinagawa-ku, Tokyo, Japan †Division for Advanced Medical Science, National Center for Child Health and Development, Tokyo, Japan ‡Division of Surgery, National Center for Child Health and Development, Tokyo, Japan §Department of Clinical Pathology, National Center for Child Health and Development, Tokyo, Japan Received January 31, 2011; final acceptance April 1, 2011.



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Heterologous protein expression and functional characterization of EAAT5. (A) AcGFP-EAAT5 expressing cells (green), counterstained with TRITC wheat germ agglutinin (red) exhibit 63% ± 6% membrane localization of EAAT5 (yellow, n = 5). (B) Immunoblot analysis reveals the typical broad electrophoretic mobility of EAATs (lane 1: nontransfected; lane 2: EAAC1-transfected; and lane 3: EAAT5-transfected cells). Molecular mass markers (lane MW) are indicated in kDa. (C) EAAT5 expression (solid squares) significantly increases L-[3H]-glutamate uptake in HEK293 cells (nontransfected HEK293, solid circles), which is in the same range as that measured for EAAT4 (19).

Journal: Biophysical Journal

Article Title: The Discovery of Slowness: Low-Capacity Transport and Slow Anion Channel Gating by the Glutamate Transporter EAAT5

doi: 10.1016/j.bpj.2011.04.034

Figure Lengend Snippet: Heterologous protein expression and functional characterization of EAAT5. (A) AcGFP-EAAT5 expressing cells (green), counterstained with TRITC wheat germ agglutinin (red) exhibit 63% ± 6% membrane localization of EAAT5 (yellow, n = 5). (B) Immunoblot analysis reveals the typical broad electrophoretic mobility of EAATs (lane 1: nontransfected; lane 2: EAAC1-transfected; and lane 3: EAAT5-transfected cells). Molecular mass markers (lane MW) are indicated in kDa. (C) EAAT5 expression (solid squares) significantly increases L-[3H]-glutamate uptake in HEK293 cells (nontransfected HEK293, solid circles), which is in the same range as that measured for EAAT4 (19).

Article Snippet: Cell culture and molecular biology HEK293 cells (No. CRL 1573; American Type Culture Collection) were cultured as described previously ( 21 ).

Techniques: Expressing, Functional Assay, Membrane, Western Blot, Transfection